Experimental study of the antibacterial efficacy of an antiseptic drug activated by ultrasound of different frequencies
https://doi.org/10.36377/1683-2981-2023-21-3-188-193
Abstract
Aim. To study the antibacterial efficacy of the antiseptic drug Miramistin, activated by ultrasound of different frequencies, against pathogenic microorganisms of periodontal pockets, in an experiment.
Materials and methods. As an antiseptic drug, the drug Miramistin 0.01% was used, which was activated using ultrasound of different frequencies: high-frequency (880 kHz) in continuous mode for 4 minutes, low-frequency (26.5 kHz) in continuous mode for 2 minutes. To conduct the study, clinical strains of microorganisms obtained from periodontal pockets with moderate periodontitis were used: S. sanguis, S. pyogenes, P. intermedia, C. albicans. The study was carried out using automatic programmable cultivation, using the RTS-8 system. The studied samples of the antiseptic drug were previously diluted in a nutrient broth in a ratio of 2:200.
Results. In the course of the study, it was found that with respect to pathogenic microorganisms obtained from periodontal pockets with moderate periodontitis, Miramistin in a dilution of 2:200, activated by low-frequency ultrasound, showed more pronounced antibacterial effect compared to Miramistin activated by high-frequency ultrasound.
Conclusions. In the treatment of periodontitis, to suppress the vital activity of pathogenic microorganisms, preference should be given to the combined effects of low-frequency ultrasound and the antiseptic drug Miramistin.
About the Authors
I. A. Paramonova
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Russian Federation
Russian Federation
Irina A. Paramonova – Postgraduate student of the Department of Therapeutic Dentistry of the Institute of Dentistry
8c2 Trubetskaya st., Moscow, 119048
A. G. Volkov
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Russian Federation
Russian Federation
Alexander G. Volkov – PhD, Professor of therapeutic dentistry department
8c2 Trubetskaya st., Moscow, 119048
N. Zh. Dikopova
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Russian Federation
Russian Federation
Natalya Zh. Dikopova – PhD, Associate professor
8c2 Trubetskaya st., Moscow, 119048
M. S. Podporin
A.I. Yevdokimov Moscow State University of Medicine and Dentistry
Russian Federation
Russian Federation
Mikhail S. Podporin – Ph.D., researcher, laboratory of molecular biological research
20с1, Delegatskaya st, Moscow, 27473
Yu. O. Paramonov
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Russian Federation
Russian Federation
Yury O. Paramonov – PhD, Associate professor
8c2 Trubetskaya st., Moscow, 119048
I. A. Nikolskaya
Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
Nikolskaya Irina Andreevna – Ph.D., Associate Professor, Department of Therapeutic Dentistry, Faculty of Dentistry
1, Ostrovityanova str., Moscow, 117997
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Review
For citations:
Paramonova I.A., Volkov A.G., Dikopova N.Zh., Podporin M.S., Paramonov Yu.O., Nikolskaya I.A. Experimental study of the antibacterial efficacy of an antiseptic drug activated by ultrasound of different frequencies. Endodontics Today. 2023;21(3):188-193. (In Russ.) https://doi.org/10.36377/1683-2981-2023-21-3-188-193
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