Биосовместимость и остеогенный потенциал багдадита, минерального триоксидного агрегата и их комбинации в отношении стволовых клеток пульпы человека: in vitro исследование

ВВЕДЕНИЕ. Стволовые клетки зубной пульпы (DPSCs) представляют интерес в регенеративной эндодонтии благодаря своей мультипотентности. Минеральный триоксидный агрегат (MTA) высоко ценится за биосовместимость, однако длительное время схватывания и трудности в обращении стимулировали интерес к новым материалам, таким как багдадит. Целью исследования было оценить биосовместимость и остеогенный потенциал багдадита, MTA и их комбинации на DPSCs с использованием МТТ-теста и окрашивания аллизарином красным.

МАТЕРИАЛЫ И МЕТОДЫ. DPSCs культивировали и характеризовали с помощью проточной цитометрии и CFU-тестов. Экспериментальные группы (MTA, багдадит, MTA+багдадит) подвергались тесту цитотоксичности (МТТ) и тесту минерализации (окрашивание аллизарином красным).

РЕЗУЛЬТАТЫ. Жизнеспособность клеток во всех группах была выше, чем в контроле. Комбинированная группа показала максимальную жизнеспособность (средняя ОП: 0,4066) по сравнению с багдадитом (0,3975) и MTA (0,3563). Окрашивание аллизарином красным продемонстрировало наибольшую минерализацию в комбинированной группе (средняя ОП: 1,7069) по сравнению с MTA (0,5788) и багдадитом (0,4020).

ВЫВОДЫ. Ассоциация MTA и багдадита показала улучшенную биосовместимость и остеогенную активность, что делает ее перспективной для применения в качестве материала для накрытия пульпы в регенеративной эндодонтии.

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