Biofilm in endodontics Part I. Properties and methods of study (review of the literature)
Abstract
About 80% of the human microbiota exists in the form of biofilms. The properties of microorganisms in the composition of biofilms differ significantly from those in planktonic suspensions. In the root canal system (RCS), the organization of microflora in biofilms has also been detected. Complex structure and individual variability of RCS in combination with unique properties of biofilm make disinfection of this system problematic. The persistence of microflora in the RCS and dentinal tubules, which continues even after qualitative clinical and radiological endodontic treatment, causes its low efficiency. In recent years, various methods for studying biofilm have been developed. The authors emphasize that the most objective of them are those that are conducted in vivo. However, it is practically impossible to implement such methods in the RCS, but methods of indirect evaluation of biofilm properties can be used. The purpose of this review is to analyze the accumulated information on the features of the biocenosis of the RCS and modern methods of studying biofilms in order to improve the effectiveness of endodontic dentistry.
About the Authors
В. Румянцев
ФГБОУ ВО «Тверской государственный медицинский университет Минздрава РФ»
Russian Federation
Russian Federation
Е. Родионова
ФГБОУ ВО «Тверской государственный медицинский университет Минздрава РФ»
Russian Federation
Russian Federation
А. Некрасов
ФГБОУ ВО «Тверской государственный медицинский университет Минздрава РФ»
Russian Federation
Russian Federation
Черджиева
ФГБОУ ВО «Тверской государственный медицинский университет Минздрава РФ»
Russian Federation
Russian Federation
М. Куприянова
ФГБОУ ВО «Тверской государственный медицинский университет Минздрава РФ»
Russian Federation
Russian Federation
References
1. Митронин А. В., Волков Д. П., Митронин В. А. Исследование диффузии озвученного антисептического водного раствора наносеребра в дентин зуба // Эндодонтия today. 2017. №2. С. 4-6.
2. Сирак С. В., Адамчик А. А., Кобылкина Т. Л., Кошель И. В., Лайпанова Ф. М. Сравнительная характеристика препаратов для временного пломбирования корневых каналов при лечении апикального периодонтита // Эндодонтия today. 2016. №3. С. 24-27.
3. Соломонов М. Е. Биопленка как эндодонтическая инфекция // Эндодонтия. 2016. Т. 9. №1-2. С. 67-69.
4. Царев В. Н., Митронин А. В., Подпорин М. С. Микробная биопленка корневых каналов и новые подходы к диагностике и лечению хронических форм пульпита с использованием фотоактивируемой дезинфекции и ультразвуковой обработки // Эндодонтия today. 2016. №3. С. 19-23.
5. Alhede M., Qvortrup K., Liebrechts R. et al. Combination of microscopic techniques reveals a comprehensive visual impression of biofilm structure and composition FEMS // Immun. Med. Microbiol. 2012. Vol. 65. Р. 335342.
6. Alves F. R., Almeida B. M., Neves M. A. et al. Disinfecting oval-shaped root canals: Effectiveness of different supplementary approaches // J. Endod. 2011. Vol. 37. Р. 496-501.
7. Alves F. R., Andrade-Junior C. V., Marceliano-Alves M. F. et al. Adjunctive steps for disinfection of the mandibular molar root canal system: A correlative bacteriologic, microcomputed tomography, and cryopulverization approach // J. Endod. 2016. Vol. 42. Р. 1667-1672.
8. Anderson A. C., Hellwig E., Vespermann R. et al. Comprehensive analysis of secondary dental root canal infections: A combination of culture and culture-independent approaches reveals new insights. // PLoS ONE. 2012. Vol. 7. e49576.
9. Antunes H. S., Rocas I. N., Alves F. R., Siqueira J. F. Total and specific bacterial levels in the apical root canal system of teeth with post-treatment apical periodontitis // J. Endod. 2015. Vol. 41. Р. 1037-1042.
10. Barbosa-Ribeiro M., De-Jesus-Soares A., Zaia A.A. et al. Antimicrobial susceptibility and characterization of virulence genes of Enterococcus faecalis isolates from teeth with failure of the endodontic treatment // J. Endod. 2016. Vol. 42. Р. 1022-1028.
11. Busscher H. J., van der Mei H. C. Microbial adhesion in flow displacement systems // Clin. Microbiol. Rev. 2006. Vol. 19. Р. 127-141.
12. Bystrom A., Sundqvist G. Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy // Scand. J. Dent. Res. 1981. Vol. 89. Р. 321-328.
13. Caggianiello G., Kleerebezem M., Spano G. Exopolysaccharides produced by lactic acid bacteria: From health-promoting benefits to stress tolerance mechanisms // Appl. Microbiol. Biotechnol. 2016. Vol. 100., Р. 3877-3886.
14. Chavez de Paz L.E. Image analysis software based on color segmentation for characterization of viability and physiological activity of biofilms // Appl. Environ. Microbiol. 2009. Vol. 75. Р. 1734-1739.
15. Chavez de Paz L. E. Development of a multispecies biofilm community by four root canal bacteria // J. Endod. 2012. Vol. 38. Р. 318-323.
16. Chavez de Paz L. E., Davies J. R., Bergenholtz G., Svensater G. Strains of Enterococcus faecalis differ in their ability to coexist in biofilms with other root canal bacteria // Int. Endod. J. 2015. Vol. 48. Р. 916-925.
17. Coenye T., de Prijck K., de Wever B., Nelis H. J. Use of the modified Robbins device to study the in vitro biofilm removal efficacy of NitrAdine, a novel disinfecting formula for the maintenance of oral medical devices // J. Appl. Microbiol. 2008. Vol. 105. Р. 733-740.
18. Costerton J.W., Stewart P.S. Battling biofilms // Sci. Am. 2001. Vol. 285. Р. 74-81.
19. Davey M. E., O’Toole G. A. Microbial biofilms: From ecology to molecular genetics // Microbiol. Mol. Biol. Rev. 2000. Vol. 64. Р. 847-867.
20. Devaraj S., Jagannathan N., Neelakantan P. Antibiofilm efficacy of photoactivated curcumin, triple and double antibiotic paste, 2% chlorhexidine and calcium hydroxide against Enterococcus fecalis in vitro // Sci. Rep. 2016. Vol. 6. Р. 24797.
21. Diaz P. I. Microbial diversity and interactions in subgingival biofilm communities // Front. Oral Biol. 2012. Vol. 15. Р. 17-40.
22. Dunavant T. R., Regan J. D., Glickman G. N. et al. Comparative evaluation of endodontic irrigants against Enterococcus faecalis biofilms // J. Endod. 2006. Vol. 32. P. 527-531.
23. Farmer J. T., Shimkevitch A. V., Reilly P. S. et al. Environmental bacteria produce abundant and diverse antibiofilm compounds // J. Appl. Microbiol. 2014. Vol. 117. P. 1663-1673.
24. Filoche S. K., Coleman M. J., Angker L., Sissons C.H. A fluorescence assay to determine the viable biomass of microcosm dental plaque biofilms // J. Microbiol. Methods. 2007. Vol. 69. P. 489-496.
25. Flemming H. C., Wingender J., Szewzyk U. et al. Biofilms: An emergent form of bacterial life // Nat. Rev. Microbiol. 2016. Vol. 14. P. 563-575.
26. Flemming H. C. EPS — then and now // Microorganisms. 2016. Vol. 4. P. 41.
27. Gabrilska R. A., Rumbaugh K. P. Biofilm models of polymicrobial infection // Future Microbiol. 2015. Vol. 10. P. 1997-2015.
28. Gerdes K., Semsey S. Microbiology: Pumping persisters // Nature. 2016. Vol. 534. P. 41-42.
29. Gilbert P., Maira-Litran T., McBain A. J. et al. The physiology and collective recalcitrance of microbial biofilm communities // Adv. Microb. Physiol. 2002. Vol. 46. P. 202-256.
30. Guo H., Gao C., Zhang C. et al. Morphology of bacterial flora in root canals associated with apical abscesses // Chin. Med. J. 2014. Vol. 127. P. 3254-3258.
31. Haapasalo M., Udnæs T., Endal U. Persistent, recurrent, and acquired infection of the root canal system post-treatment // Endod. Top. 2003. Vol. 6. P. 29-56.
32. He Z., Liang J., Zhou W., Xie Q., Tang Z., Ma R., Huang Z. Effect of the quorum-sensing luxS gene on biofilm formation by Enterococcus faecalis // Eur. J. Oral Sci. 2016. Vol. 124. P. 234-240.
33. Honraet K., Nelis H. J. Use of the modified Robbins device and fluorescent staining to screen plant extracts for the inhibition of S. mutans biofilm formation // J. Microbiol. Methods. 2006. Vol. 64. P. 217-224.
34. Kaldalu N., Hauryliuk V., Tenson T. Persisters - as elusive as ever // Appl. Microbiol. Biotechnol. 2016. Vol. 100. P. 6545-6553.
35. Kelleher S. M., Habimana O., Lawler J. et al. Cicada wing surface topography: an investigation into the bactericidal properties of nanostructural features // ACS Appl. Mater. Interfaces. 2016. Vol. 8. P. 14966-14974.
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