Актуальность применения хлоргексидина в адгезивном протоколе в девитальных зубах

Цель. Определение актуальности применения хлоргексидинового адгезивного протокола в девитальных зубах.
Материалы и методы. Был проведен систематический обзор литературы в электронных базах данных Google Scholar и Pubmed. Рассмотрены и включены статьи, касающиеся исследований активности матриксных металлопротеиназ в витальных и в девитальных зубах, а также исследования об эффективности хлоргексидинового протокола.
Результаты. В ходе обзора было рассмотрено 60 статьи. После произведения отбора по критериям исключения, число включенных исследований составило 36.
Выводы. По данным изученных публикаций, было установлено, что ММП 2 и ММП 9 содержатся не только в витальных, но и в девитальных зубах. Применение хлоргексидинового адгезивного протокола актуально и в том, и в другом клиническом случае, однако в зависимости от вида ММП и состояния пульпы подбирается концентрация хлоргексидина.

1. Chaussain-Miller C., Fioretti F., Goldberg M., Menashi S. The role of matrix metalloproteinases (MMPs) in human caries. J Dent Res 2006; 85:22–32.

2. Cieplak P., Strongin A.Y./ Matrix metalloproteinases – From the cleavage data to the prediction tools and beyond. Biochim Biophys Acta. 2017. pii: S0167-4889(17)30064-23. Raffetto J.D., Khalil R.A./ Matrix metalloproteinases and their inhibitors in vascular remodeling and vascular disease. Biochem Pharmacol. 2008;75(2):346–59

3. Lu Y, Papagerakis P, Yamakoshi Y, Hu JC, Bartlett JD, Simmer JP. Functions of KLK4 and MMP-20 in dental enamel formation. Biol Chem. 2008 Jun;389(6):695-700.

4. Prajapati S, Tao J, Ruan Q, De Yoreo JJ, Moradian-Oldak J Matrix metalloproteinase-20 mediates dental enamel biomineralization by preventing protein occlusion inside apatite crystals. Journal Biomaterials. 2016; 75: 260-270.

5. Vladimirova M. D.,Veselkov S. A. Matrix metalloproteinases of the tooth Scientific journal "Student forum". 2019; 770 (26): 13-15.

6. Abdulkerimova SM, Khabadze Z.S. "Clinical experience of the use of aqueous and alcohol solutions of chlorhexidine in the treatment of dentin caries" Scientific discussion. 2018; 17(1).

7. Ganusevich I. I. Oncology. 2010; 1 (1): 10-16

8. Tezvergil-Mutluay A., Mutluay M.M., Gu L.S., Zhang K., Agee K.A., Carvalho R.M., et al. The anti MMP activity of benzalkonium chloride. J Dent. 2011; 39: 57-64.

9. Gendron R., Grenier D., Sorsa T., Mayrand D. Inhibition of the activities of matrix metalloproteinases 2, 8, and 9 by chlorhexidine. Clin Diagn Lab Immunol 1999; 6: 437-439.

10. De Munck J., Van den Steen P.E., Mine A., Van Landuyt K.L., Poitevin A., Opdenakker G., et al. Inhibition of enzymatic degradation of adhesive-dentin interfaces. J Dent Res 2009;88: 1101-1106.

11. P.C.Moon, J.Weaver, C.N.Brooks . Review of Matrix Metalloproteinases’ Eff ect on the Hybrid Dentin Bond Layer Stability and Chlorhexidine Clinical Use to Prevent Bond Failure The Open Dent J. 2010; 4 (3): 147-152.

12. I. Ramezanian, E. Baradaran Effect of Chlorhexidine and Ethanol on Microleakage of Composite Resin Restoration to Dentine. The Chinese Journal of Dental Research 2017; 20: 161 -168.

13. Freeman, D., And Leinfelder, K. Seventh-generation adhesive systems. Clinical dentistry. 2003; 3: 4-8.

14. Gregoire G. Microscopic evaluation of dentin interface obtained with 10 contemporary self-etching systems: correlation with their pH. Oper Dent. 2005 Jul Aug N.30(4)

15. Luz M.A., Arana-Chavez V.E., Netto N.G.Scanning electron microscopy examination of 3 different adhesive systems. Quintessence Int. 2005 N.36(9)

16. Breschi L., Mazzoni A., Nato F., Carrilho M., Visintini E., Tjäderhane L., Ruggeri A. Jr., Tay F. R., Dorigo Ede S., Pashley D. H.Chlorhexidine stabilizes the adhesives interface: a 2-year in vitro study. Dent Mater 2009.

17. Carrilho M. R., Geraldeli S., Tay F., de Goes M. F., Carvalho R. M., Tjäderhane L, Reis A.F., Hebling J., Mazzoni A., Breschi L., Pashley D. In vivo preservation of the hybrid layer by chlorhexidine. J Dent Res. 2007

18. Shimada Y, Ichinose S, Sadr A, Burrow MF, Tagami J. Localization of matrix metalloproteinases (MMPs-2, 8, 9 and 20) in normal and carious dentine. Aust Dent J. 2009 Dec;54(4):347-54.

19. Sorsa T., Suomalainen K., Uitto V.J. The role of gingival crevicular fluid and salivary interstitial collagenases in human periodontal diseases. Arch Oral Biol 1990;35(Suppl):193S–196S

20. Rai B, Kharb S, Jain R, Anand SC. Biomarkers of periodontitis in oral fluids. J Oral Sci. 2008 Mar;50(1):53-6.

21. Sloan A.J., Smith A.J. Stimulation of the dentine-pulp complex of rat incisor teeth by transforming growth factor-b isoforms 1-3 in vitro. Arch Oral Biol 1999;44:149–156.

22. Niu LN, Zhang L, Jiao K, Li F, Ding YX, Wang DY, Wang MQ, Tay FR, Chen JH. Localization of MMP-2, MMP-9, TIMP-1, and TIMP-2 in human coronal dentine. J Dent. 2011 Aug;39(8):536-42

23. Lu KV, Jong KA, Rajasekaran AK, Cloughesy TF, Mischel PS.Upregulation of tissue inhibitor of metalloproteinases (TIMP)- 2 promotes matrix metalloproteinase (MMP)-2 activation and cell invasion in a human glioblastoma cell line. Laboratory Investigation 2004; 84:8–20

24. Nagase H., Woessner J.F. Jr. Matrix metalloproteinases. J Biol Chem; 274: 21491–21494. 1999.

25. Kushlinskii N.E., Solovykh E.A., Karaoglanova T.B., Bayar U., Gershtein E.S., Troshin A.A., Kostyleva O.I., Grinin V.M., Maksimovskaya L.N., Yanushevitch O.O. Content of matrix metalloproteinase-8 and matrix metalloproteinase-9 in oral fluid of patients with chronic generalized periodontitis. Bull Exp Biol Med; 15: 240-244 .2011.

26. Boushell L.W., Swift E.J. Jr. Critical appraisal. Dentin bonding: matrixmetalloproteinases and chlorhexidine. J Esthet Restor Dent; 23: 347-352.2011.

27. Breschi L., Martin P., Mazzoni A., Nato F., Carrilho M., Tjäderhane L., Visintini E, Cadenaro M., Tay F.R., De Stefano Dorigo E., Pashley D.H. Use of a specific MMP-inhibitor (galardin) for preservation of hybrid layer. Dent Mater; 26: 571-578. 2010.

28. Hebling J., Pashley D.H., Tjäderhane L., Tay F.R.Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo.J Dent Res; 84: 741-746.2005.

29. Gendron R., Grenier D., Sorsa T.,Mayrand D. Inhibition of the activities of matrix metalloproteinases 2, 8 and 9 by chlorhexidine. Clin Diagn Lab Immunol 1999;6: 437 –439.

30. Trufello AM, Orellana BU, Moraga CL, Puente CL, Morales-Bozo I. Subclinical concentrations of chlorhexidine inhibit gelatinase activity of carious dentine in vitro. Aust Dent J. 2014 Mar;59(1):81-6.

31. R. Gendron, D. Grenier, T. Sorsa, D. Mayrand. Inhibition of the Activities of Matrix Metalloproteinases 2, 8 and 9 by Chlorhexidine. C LINICAL AND D IAGNOSTIC L ABORATORY I MMUNOLOGY. May 1999 p. 437–439

32. A. Mazzoni, F. Mannello, F.R. Tay, G.A.M. Tonti, S. Papa, G. Mazzotti, R. Di Lenarda, D.H. Pashley, L. Breschi. Zymographic Analysis and Characterization of MMP-2 and -9 Forms in Human Sound Dentin. J Dent Res -86(5):436-440, 2007

33. de Souza AP, Gerlach RF, Line SR. Inhibition of human gingival gelatinases (MMP-2 and MMP-9) by metal salts. Dent Mater. 2000 Mar;16(2):103-8.

34. Alaghehmad H, Mansouri E, Esmaili B, Bijani A, Nejadkarimi S, Rahchamani M. Effect of 0.12% chlorhexidine and zinc nanoparticles on the microshear bond strength of dentin with a fifth-generation adhesive. Eur J Dent. 2018;12(1):105-110.

35. Osorio R, Yamauti M, Osorio E, Román JS, Toledano M. Zinc-doped dentin adhesive for collagen protection at the hybrid layer. Eur J Oral Sci. 2011 Oct;119(5):401-10.