Preview

Endodontics Today

Advanced search

Ex vivo study of the efficacy of two irrigation systems in retreatment of root canals filled with bioceramic sealers

https://doi.org/10.36377/ET-0112

Abstract

AIM. To evaluate the presence of residual filling material in the root canal walls after retreatment and final irrigation using scanning electron microscopy (SEM).

MATERIALS AND METHODS. Fifty-two mandibular oval premolars were instrumented with ProTaper Next X1–X3 and divided into 2 groups: Group BC, filled with Bio-C Sealer and Group AH, filled with AH Plus. The experimental groups were retreated with a ProTaper Next X4 file and received different irrigation protocols (n = 13): Group BC-PUI: agitation with ultrasound; Group BC-EC: agitation with Easy Clean; Group AH-PUI: agitation with ultrasound; and Group AH-EC: agitation with Easy Clean. The remaining filling material in the dentinal tubules was evaluated in scanning electron microscope images by three evaluators who scored the samples. The Kappa test was performed to check interexaminer agreement. Pearson’s chi-square test with a significance level < of 5% was performed to compare the groups.

RESULTS. It was found that the BC-EC group showed better results in the apical and middle thirds. In the coronal third, the BC-PUI group also performed well. AH Plus showed the lower results in all situations.

CONCLUSIONS. In the apical third, the BC-EC group showed better performance, in the middle third, the AH-PUI group had the worst performance, and in the coronal third, the BC-PUI group stood out in terms of cleaning. This manuscript provides the importance of thorough cleaning, especially in difficult areas such as the apical third, is emphasized to improve the overall outcome of treatment

About the Authors

D.C.A. Freire
Instituto de Pesquisas São Leopoldo Mandic
Brazil

Danielle Cardoso Albuquerque Maia Freire – Dentist, MSc holding a Master’s degree in Endodontics, Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Department of Endodontics

Campinas, São Paulo, Brazil


Competing Interests:

The authors declare no conflict of interests.



R. A. Pelegrine
Instituto de Pesquisas São Leopoldo Mandic
Brazil

Rina Andrea Pelegrine – Dentist, MSc, PhD and Professor in the Department of Endodontics, Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Department of Endodontics

Campinas, São Paulo, Brazil


Competing Interests:

The authors declare no conflict of interests.



D.G.P. Rocha
PUC Campinas
Brazil

Daniel Guimarães Pedro Rocha – Dr. Sci. (Med.), Lecturer and Researcher in the Department of Endodontics at the Faculty of Dentistry, PUC Campinas, Department of Endodontics, Center of Life Sciences, Programa de pós-graduação em Ciências da Saúde

Campinas, São Paulo, Brazil


Competing Interests:

The authors declare no conflict of interests.



W. M. Nascimento
Instituto de Pesquisas São Leopoldo Mandic
Brazil

Wayne Martins Nascimento – Dentist, MSc, PhD and Professor and Researcher Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Departament of Endodontics

Campinas, São Paulo, Brazil


Competing Interests:

The authors declare no conflict of interests.



A.G.S. Limoeiro
University of Sao Paulo
Brazil

Ana Grasiela da Silva Limoeiro – Dentist, MSc, PhD and Professor, Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School

Bauru, Brazil


Competing Interests:

The authors declare no conflict of interests.



E.F.V. Marceliano
Brazilian Army General Hospital of Belem
Brazil

Eduardo Fagury Videira Marceliano – Dentist, Master in Dentistry, lecturer and researcher Dental Clinic Department

Belem, Brazil


Competing Interests:

The authors declare no conflict of interests.



A.R.L.S. Miranda
University Center of Pará
Brazil

Ana Raquel Lopes dos Santos Miranda – Dentist, MSc and Professor, Department of Dentistry

Belém, Brazil


Competing Interests:

The authors declare no conflict of interests.



T.M.C. Coutinho
Dr. D.Y. Patil Dental College and Hospital
India

Thais Machado de Carvalho Coutinho – Dentist, MSc, PhD and Professor in the Department of Dentistry, Iguaçu University, Postgraduate Program in Dentistry

Nova Iguaçu, RJ, Brazil


Competing Interests:

The authors declare no conflict of interests.



M.F.V. Marceliano-Alves
Dr. D.Y. Patil Dental College and Hospital; Maurício de Nassau University Centre (UNINASSAU); Iguaçu University
Brazil

Marilia Fagury Videira Marceliano-Alves – Dentist, Holds MSc and PhD degrees in Endodontics, Professor and Researcher, Professor at Posrgraduate Program in Dentistry

Nova Iguaçu, Brazil


Competing Interests:

The authors declare no conflict of interests.



C.E.B. Bueno
Instituto de Pesquisas São Leopoldo Mandic
Brazil

Carlos Eduardo da Silveira Bueno – Dentist, MSc, PhD and Professor, Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Departament of Endodontics

Campinas, São Paulo, Brazil


Competing Interests:

The authors declare no conflict of interests.



References

1. Siqueira J.F. Jr, Rôças I.N. Clinical implications and microbiology of bacterial persistence after treatment procedures. J Endod. 2008;34(11):1291–1301.e3. https://doi.org/10.1016/j.joen.2008.07.028

2. Rossi-Fedele G., Ahmed H.M. Assessment of root canal filling removal effectiveness using micro-computed tomography: A systematic review. J Endod. 2017;43(4):520–526. https://doi.org/10.1016/j.joen.2016.12.008

3. Silva E.J.N.L., Belladonna F.G., Zuolo A.S., Rodrigues E., Ehrhardt I.C., Souza E.M., De-Deus G. Effectiveness of XP-endo Finisher and XP-endo Finisher R in removing root filling remnants: a micro-CT study. Int Endod J. 2018;51(1):86–91. https://doi.org/10.1111/iej.12788

4. van der Sluis L.W., Vogels M.P., Verhaagen B., Macedo R., Wesselink P.R. Study on the influence of refreshment/activation cycles and irrigants on mechanical cleaning efficiency during ultrasonic activation of the irrigant. J Endod. 2010;36(4):737–740. https://doi.org/10.1016/j.joen.2009.12.004

5. Rodrigues R.C.V., Zandi H., Kristoffersen A.K., Enersen M., Mdala I., Ørstavik D. et al. Influence of the apical preparation size and the irrigant type on bacterial reduction in root canal-treated teeth with apical periodontitis. J Endod. 2017;43(7):1058–1063. https://doi.org/10.1016/j.joen.2017.02.004

6. Duque J.A., Duarte M.A., Canali L.C., Zancan R.F., Vivan R.R., Bernardes R.A., Bramante C.M. Comparative effectiveness of new mechanical irrigant agitating devices for debris removal from the canal and isthmus of mesial roots of mandibular molars. J Endod. 2017;43(2):326–331. https://doi.org/10.1016/j.joen.2016.10.009

7. Nevares G., de Albuquerque D.S., Freire L.G., Romeiro K., Fogel H.M., Dos Santos M., Cunha R.S. Efficacy of ProTaper NEXT compared with reciproc in removing obturation material from severely curved root canals: A micro-computed tomography study. J Endod. 2016;42(5):803–808. https://doi.org/10.1016/j.joen.2016.02.010

8. Bago I., Suk M., Katić M., Gabrić D., Anić I. Comparison of the effectiveness of various rotary and reciprocating systems with different surface treatments to remove gutta-percha and an epoxy resin-based sealer from straight root canals. Int Endod J. 2019;52(1):105–113. https://doi.org/10.1111/iej.12985

9. Wright C.R., Glickman G.N., Jalali P., Umorin M. Effectiveness of gutta-percha/sealer removal during retreatment of extracted human molars using the gentlewave system. J Endod. 2019;45(6):808–812. https://doi.org/10.1016/j.joen.2019.02.009 (Erratum in: J Endod. 2019;45(8):1060. https://doi.org/10.1016/j.joen.2019.05.019)

10. Chybowski E.A., Glickman G.N., Patel Y., Fleury A., Solomon E., He J. Clinical outcome of non-surgical root canal treatment using a single-cone technique with endosequence bioceramic sealer: A retrospective analysis. J Endod. 2018;44(6):941–945. https://doi.org/10.1016/j.joen.2018.02.019 (Erratum in: J Endod. 2018;44(7):1199. https://doi.org/10.1016/j.joen.2018.06.001)

11. Silva Almeida L.H., Moraes R.R., Morgental R.D., Pappen F.G. Are premixed calcium silicate-based endodontic sealers comparable to conventional materials? A systematic review of in vitro studies. J Endod. 2017;43(4):527–535. https://doi.org/10.1016/j.joen.2016.11.019

12. Kim H., Kim E., Lee S.J., Shin S.J. Comparisons of the retreatment efficacy of calcium silicate and epoxy resin-based sealers and residual sealer in dentinal tubules. J Endod. 2015;41(12):2025–2030. https://doi.org/10.1016/j.joen.2015.08.030

13. Bernardes R.A., Duarte M.A.H., Vivan R.R., Alcalde M.P., Vasconcelos B.C., Bramante C.M. Comparison of three retreatment techniques with ultrasonic activation in flattened canals using micro-computed tomography and scanning electron microscopy. Int Endod J. 2016;49(9):890–897. https://doi.org/10.1111/iej.12522

14. Chen X., Liu H., He Y., Luo T., Zou L. Effects of endodontic sealers and irrigation systems on smear layer removal after post space preparation. J Endod. 2018;44(8):1293–1297. https://doi.org/10.1016/j.joen.2018.05.014

15. Volponi A., Pelegrine R.A., Kato A.S., Stringheta C.P., Lopes R.T., Silva A.S.S., Bueno C.E.D.S. Micro-computed tomographic assessment of supplementary cleaning techniques for removing bioceramic sealer and guttapercha in oval canals. J Endod. 2020;46(12):1901–1906. https://doi.org/10.1016/j.joen.2020.09.010

16. Schneider S.W. A comparison of canal preparations in straight and curved root canals. Oral Surg Oral Med Oral Pathol. 1971;32(2):271–275. https://doi.org/10.1016/0030-4220(71)90230-1

17. Kato A.S., Cunha R.S., da Silveira Bueno C.E., Pelegrine R.A., Fontana C.E., de Martin A.S. Investigation of the efficacy of passive ultrasonic irrigation versus irrigation with reciprocating activation: An environmental scanning electron microscopic study. J Endod. 2016;42(4):659–663. https://doi.org/10.1016/j.joen.2016.01.016

18. Pirani C., Pelliccioni G.A., Marchionni S., Montebugnoli L., Piana G., Prati C. Effectiveness of three different retreatment techniques in canals filled with compacted gutta-percha or Thermafil: A scanning electron microscope study. J Endod. 2009;35(10):1433–1440. https://doi.org/10.1016/j.joen.2009.06.002

19. Martins M.P., Duarte M.A., Cavenago B.C., Kato A.S., da Silveira Bueno C.E. Effectiveness of the ProTaper next and reciproc systems in removing root canal filling material with sonic or ultrasonic irrigation: A micro-computed tomographic study. J Endod. 2017;43(3):467–471. https://doi.org/10.1016/j.joen.2016.10.040

20. Penha da Silva P.J., Marceliano-Alves M.F., Provenzano J.C., Dellazari R.L.A., Gonçalves L.S., Alves F.R.F. Quality of root canal filling using a bioceramic sealer in oval canals: A three-dimensional analysis. Eur J Dent. 2021;15(3):475–480. https://doi.org/10.1055/s-0040-1722095

21. Tavares S.J.O., Gomes C.C., Marceliano-Alves M.F., Guimarães L.C., Provenzano J.C., Amoroso-Silva P. et al. Supplementing filling material removal with XP-Endo Finisher R or R1-Clearsonic ultrasonic insert during retreatment of oval canals from contralateral teeth. Aust Endod J. 2021;47(2):188–194. https://doi.org/10.1111/aej.12451

22. Ajina M.A., Shah P.K., Chong B.S. Critical analysis of research methods and experimental models to study removal of root filling materials. Int Endod J. 2022;55(Suppl. 1):119–152. https://doi.org/10.1111/iej.13650

23. Horvath S.D., Altenburger M.J., Naumann M., Wolkewitz M., Schirrmeister J.F. Cleanliness of dentinal tubules following gutta-percha removal with and without solvents: A scanning electron microscopic study. Int Endod J. 2009;42(11):1032–1038. https://doi.org/10.1111/j.1365-2591.2009.01616.x

24. Huang Y., Orhan K., Celikten B., Orhan A.I., Tufenkci P., Sevimay S. Evaluation of the sealing ability of different root canal sealers: a combined SEM and micro-CT study. J Appl Oral Sci. 2018;26:e20160584. https://doi.org/10.1590/1678-7757-2016-0584

25. de Souza D.S., S Silva A.S., Ormiga F., Lopes R.T., Gusman H. The effectiveness of passive ultrasonic irrigation and the easy-clean instrument for removing remnants of filling material. J Conserv Dent. 2021;24(1):57–62. https://doi.org/10.4103/JCD.JCD_590_20

26. Colombo J.A., Rocha D.G., Limoeiro A.S., Nascimento W.M., Fontana C.E., Pelegrine R.A. et al. Micro-CT evaluation of sealers removal by reciprocal instrumentation followed by continuous ultrasonic irrigation in teeth with oval root canals. J Clin Exp Dent. 2023;15(3):e233–e238. https://doi.org/10.4317/jced.60013

27. Madarati A.A., Sammani A.M.N., Alnazzawi A.A., Alrahlah A. Efficiency of the new reciprocating and rotary systems with or without ultrasonics in removing rootcanals filling with calcium silicate-based sealer (MTA). BMC Oral Health. 2023;23(1):5. https://doi.org/10.1186/s12903-022-02684-3

28. Eymirli A., Sungur D.D., Uyanik O., Purali N., Nagas E., Cehreli Z.C. Dentinal tubule penetration and retreatability of a calcium silicate-based sealer tested in bulk or with different main core material. J Endod. 2019;45(8):1036–1040. https://doi.org/10.1016/j.joen.2019.04.010

29. Zordan-Bronzel C.L., Torres F.F.E., Tanomaru-Filho M., Chávez-Andrade G.M., Bosso-Martelo R., Guerreiro- Tanomaru J.M. Evaluation of physicochemical properties of a new calcium silicate-based sealer, Bio-C Sealer. J Endod. 2019;45(10):1248–1252. https://doi.org/10.1016/j.joen.2019.07.006

30. Kaloustian M.K., Hachem C.E., Zogheib C., Nehme W., Hardan L., Rached P. et al. Effectiveness of the revision system and sonic irrigation in the removal of root canal filling material from oval canals: An in vitro study. Bioengineering. 2022;9(6):260. https://10.3390/bioengineering9060260


Review

For citations:


Freire D., Pelegrine R.A., Rocha D., Nascimento W.M., Limoeiro A., Marceliano E., Miranda A., Coutinho T., Marceliano-Alves M., Bueno C. Ex vivo study of the efficacy of two irrigation systems in retreatment of root canals filled with bioceramic sealers. Endodontics Today. 2025;23(3):399-407. https://doi.org/10.36377/ET-0112



Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 1683-2981 (Print)
ISSN 1726-7242 (Online)