Scanning electron microscopy studies of biofilm in teeth with chronic apical periodontitis
V. V. Glinkin,
I. V. Chaikovskaya,
P. A. Kondratyev,
M. A. Gasbanov,
N. N. Glushchenko,
D. A. Babakhanov https://doi.org/10.36377/ET-0118
Abstract
INTRODUCTION. For a long time, the inflammatory process can be asymptomatic in the periapical space. Apical periodontitis is often associated with the presence of biofilm. The latter has a great influence on the prognosis of endodontic treatment.
AIM. Using scanning electron microscopy, to determine the presence and localization of biofilm and microflora in the roots of teeth with chronic apical periodontitis.
MATERIALS AND METHODS. Grins of 6 roots of teeth with chronic forms of apical periodontitis were studied for detection of microflora using the scanning electron microscopy method.
RESULTS. In 100% of cases, biofilm was found in the root canals of teeth with chronic forms of apical periodontitis. It was located focally, unevenly capturing different areas of both the root canal and the surrounding dentin of the tooth root.
DISCUSSION. Biofilm is observed in the necrotic dentin of the cervical third of the root canal, covers the pulp tissue of the tooth, penetrates into the thickness of the dentin. Microflora fills the lumen of the dentinal tubules, but does not penetrate through the intact cementum of the tooth root.
CONCLUSIONS. Microflora in the root canals of teeth with chronic forms of apical periodontitis is present not only in the form of individual cultures, but mainly in the form of biofilm, which covers not only the canal, but also penetrates to different depths into the thickness of the dentin of the tooth root. Biofilm covers dentin unevenly in different parts of the root.
About the Authors
V. V. GlinkinRussian Federation
Vladimir V. Glinkin – Dentist, Private Dental Practice
Competing Interests:
The authors report no conflict of interest.
I. V. Chaikovskaya
Russian Federation
Ilona V. Chaikovskaya – Dr. Sci. (Med.), Professor, Dentist, Private Dental Practice
Competing Interests:
The authors report no conflict of interest.
P. A. Kondratyev
Russian Federation
Pavel A. Kondratyev – Dentist, Private Dental Practice
Competing Interests:
The authors report no conflict of interest.
M. A. Gasbanov
Peoples’ Friendship University of Russia named after Patrice Lumumba (RUDN University)
Russian Federation
Competing Interests:
Russian Federation
Magomed-Ali A. Gasbanov – Assistant Lecturer, Department of Therapeutic Dentistry
6 Miklukho-Maklaya Str., Moscow 117198, Russian Federation
Competing Interests:
The authors report no conflict of interest.
N. N. Glushchenko
Peoples’ Friendship University of Russia named after Patrice Lumumba (RUDN University)
Russian Federation
Competing Interests:
Russian Federation
Nataliya N. Glushchenko – Assistant Lecturer, Department of Therapeutic Dentistry
6 Miklukho-Maklaya Str., Moscow 117198, Russian Federation
Competing Interests:
The authors report no conflict of interest.
D. A. Babakhanov
Peoples’ Friendship University of Russia named after Patrice Lumumba (RUDN University)
Russian Federation
Competing Interests:
Russian Federation
David A. Babakhanov – Postgraduate Student, Department of Therapeutic Dentistry
6 Miklukho-Maklaya Str., Moscow 117198, Russian Federation
Competing Interests:
The authors report no conflict of interest.
References
1. Mitronin A.V., Gerasimova M.M. Endodontic treatment of a pulpal and periodontal diseases (part 1). Aspects of application of antibacterial agents. Endodontics Today. 2012;10(1):9–15. (In Russ.) Available at: https://www.endodont.ru/jour/article/view/700 (accessed: 12.06.2025).
2. Kovaleva I.P., Ketova G.G. Clinical and economic analysis of the treatment of chronic apical periodontitis. Endodontics Today. 2011;9(4):47–50. (In Russ.) Available at: https://www.endodont.ru/jour/article/view/724 (accessed: 12.06.2025).
3. Kukushkin V.L., Smirnitskaya M.V., Kukushkina E.A., Nikulina V.Yu. Epidemiological aspects of caries complications. Endodontics Today. 2014;12(1):3–5. (In Russ.) Available at: https://www.endodont.ru/jour/article/view/508 (accessed: 12.06.2025).
4. Zehnder M., Belibasakis G.N. On the dynamics of root canal infections-what we understand and what we don’t. Virulence. 2015;6(3):216–222. https://doi.org/10.4161/21505594.2014.984567
5. Sobieszczański J., Mertowski S., Sarna-Boś K., Stachurski P., Grywalska E., Chałas R. Root canal infection and its impact on the oral cavity microenvironment in the context of immune system disorders in selected diseases: A narrative review. J Clin Med. 2023;12(12):4102. https://doi.org/10.3390/jcm12124102
6. Serrage H.J., Jepson M.A., Rostami N., Jakubovics N.S., Nobbs A.H. Understanding the matrix: The role of extracellular DNA in oral biofilms. Front Oral Health. 2021;2:640129. https://doi.org/10.3389/froh.2021.640129
7. Glinkin V.V., Klemin V.A., Chaikovskaya I.V., Kondratyev P.A., Komarevskaya E.V. Study of microbial contamination of root canals in apical periodontitis. University Clinic. 2024;(4):66–69. (In Russ.).
8. Siqueira J.F. Jr, Rôças I.N., Ricucci D. Biofilms in endodontic infection. Endod Topics. 2010;22(1):33–49. https://doi.org/10.1111/j.1601-1546.2012.00279.x
9. Ramirez-Mora T., Retana-Lobo C., Valle-Bourrouet G. Biochemical characterization of extracellular polymeric substances from endodontic biofilms. PLoS ONE. 2018;13(11):e0204081. https://doi.org/10.1371/journal.pone.0204081
10. Gerasimova L.P., Aletdinova S.M. Diagnostics and complex treatment of inveterate apical periodontitis in acute condition. Endodontics Today. 2014;12(1):6–9. (In Russ.) Available at: https://www.endodont.ru/jour/article/view/509 (accessed: 12.06.2025).
11. Siqueira J.F. Jr, Rôças I.N. Bacterial pathogenesis and mediators in apical periodontitis. Braz Dent J. 2007;18(4):267–280. https://doi.org/10.1590/s0103-64402007000400001
Review
For citations:
Glinkin V.V., Chaikovskaya I.V., Kondratyev P.A., Gasbanov M.A., Glushchenko N.N., Babakhanov D.A. Scanning electron microscopy studies of biofilm in teeth with chronic apical periodontitis. Endodontics Today. https://doi.org/10.36377/ET-0118
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